April 12-18, 2021
Charleston, SC
Seed | Name | Country |
| Katerina STEWART | USA | |
| Fernanda CONTRERAS GOMEZ | MEX | |
| 4 | Natalia VIKHLYANTSEVA | RUS |
| Alycia PARKS | USA | |
| 5 | Caroline DOLEHIDE | USA |
| 3 | Xinyu WANG | CHN |
| 7 | Magdalena FRECH | POL |
| Katherine SEBOV | CAN | |
| Grace MIN | USA | |
| Gabriela TALABA | ROU | |
| Oksana KALASHNIKOVA | GEO | |
| Ellen PEREZ | AUS | |
| Mirjam BJORKLUND | SWE | |
| Kennedy SHAFFER | USA | |
| Katrina SCOTT | USA | |
| Varvara FLINK | RUS | |
| Lucie HRADECKA | CZE | |
| 8 | Kurumi NARA | JPN |
| 1 | Harriet DART | GBR |
| Catherine HARRISON | USA | |
| 10 | Allie KIICK | USA |
| Whitley PATE | USA | |
| Rachel GAILIS | USA | |
| 16 | Kateryna BONDARENKO | UKR |
| Jamie LOEB | USA | |
| 6 | Whitney OSUIGWE | USA |
| Gabriela DABROWSKI | CAN | |
| 2 | Kristina KUCOVA | SVK |
| 12 | Asia MUHAMMAD | USA |
| Alexa GUARACHI | USA | |
| 11 | Storm SANDERS | CAN |
| Desirae KRAWCZYK | USA |